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Thiol-disulfide status and acridine orange fluorescence of mammalian sperm nuclei

Undetectable Intracellular Free Copper: The Requirement of a Copper Chaperone for Superoxide Dismutase

Chiang AN, Wu HL, Yeh HI, Chu CS, Lin HC, Lee WC (2006) Antioxidant effects of black rice extract through the induction of superoxide dismutase and catalase activities

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After incubation for 15 min at 30 C, 1,000 rpm using a 3 mm shaking diameter, cell debris was removed by centrifugation (4,400 g at 4 C for 30 min) and the supernatants were analysed by HPLCMS at two different dilutions (undiluted and four times diluted with 67% methanol, 0.2% hydrochloric acid in H 2 O)